eprintid: 1741 rev_number: 12 eprint_status: archive userid: 6 dir: disk0/00/00/17/41 datestamp: 2016-09-15 12:23:00 lastmod: 2017-02-08 12:21:44 status_changed: 2016-09-15 12:23:00 type: article metadata_visibility: show creators_name: Zhang, Yanyan creators_name: McPhedran, Kerry N. creators_name: Gamal El-Din, Mohamed corp_creators: Department of Civil and Environmental Engineering, University of Alberta, Canada corp_creators: Department of Civil and Environmental Engineering, University of Alberta, , Canada corp_creators: Department of Civil and Environmental Engineering, University of Alberta, CAnada title: Pseudomonads biodegradation of aromatic compounds in oil sands process-affected water subjects: O subjects: RCA1 subjects: SHU subjects: SZ divisions: SHEER full_text_status: none keywords: Aromatics; Biodegradation; Oil sands process-affected water; Pseudomonads; Toxicity abstract: Aromatic naphthenic acids (NAs) have been shown to be more toxic than the classical NAs found in oil sands process-affected water (OSPW). To reduce this toxicity, Pseudomonas fluorescens and Pseudomonas putida were used to determine their ability to biodegrade aromatic compounds including treatments considering the impacts of external carbon and iron addition. Results showed that with added carbon P. fluorescens and P. putida have the capability of biodegrading these aromatics. In the presence of external carbon, gene expression of a functional PAH-ring hydroxylating dioxygenase (PAH-RHDα) was determined through reverse transcription real-time PCR, suggesting active degradation of OSPW aromatic compounds. Although no significant classical NAs removal was observed during this process, toxicity was reduced by 49.3% under optimal conditions. OSPW toxicity was eliminated with the combination of ozonation at a dose of 80 mg/L followed by biodegradation, indicating that it is a promising combined OSPW treatment approach for the safe discharge to the aquatic environment. date: 2015-06 date_type: published publication: Science of The Total Environment volume: 521-52 publisher: Elsevier pagerange: 59-67 id_number: doi:10.1016/j.scitotenv.2015.03.068 issn: 0048-9697 official_url: http://doi.org/10.1016/j.scitotenv.2015.03.068 access_IS-EPOS: limited owner: Publisher citation: Zhang, Yanyan and McPhedran, Kerry N. and Gamal El-Din, Mohamed (2015) Pseudomonads biodegradation of aromatic compounds in oil sands process-affected water. Science of The Total Environment, 521-52. pp. 59-67. DOI: https://doi.org/10.1016/j.scitotenv.2015.03.068